Ecl Western Blot Detection System

Ecl western blotting system uses horseradish peroxidase hrp conjugated anti mouse and anti rabbit antibodies for luminol based detection of western blots.

Ecl western blot detection system.

Pierce ecl western blotting substrate provides reliability and performance equivalent to other standard ecl substrates for detection of hrp enzyme activity. Improving the overall efficiency of electrophoresis systems these reagents. Expression and purification of gst tagged proteins can be monitored by western blot analysis using amersham ecl amersham ecl prime or amersham ecl select detection systems to enhance sensitivity. Detection of gst tagged proteins with western blot.

Amersham ecl western blotting system is designed for detection of mouse and rabbit primary antibodies on western blots using hrp conjugated secondary antibodies and ecl reagentsnucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Contents include the following. Gerpn2109 62 5 ml each of ecl detection reagents 1 and 2 1000 cm 2 gerpn2209 125 ml each 2000 cm 2 gerpn2106 250 ml each 4000 cm 2 gerpn2134 375 ml. Blots probed with mouse or rabbit primary antibodies are incubated with hrp conjugated.

Ecl substrates for high sensitivity western blot detection chemiluminescent assays depend on the emission of light as a product of a chemical reaction and are commonly used for the detection of proteins on western blots. Each system contains the following reagents sufficient for detection of 1000 cm membrane. Ecl western blotting detection reagents in combination with hybond ecl membranes form a complete solution for western blot applications. Anti mouse hrp conjugate 100 µl anti rabbit hrp conjugate 100 µl ecl detection reagents 1 and 2 62 5 ml each blocking agent 5 g.